GeneBank序列注释说明
GeneBank格式的序列中,有一些注释很难弄明白它的意思,通过搜索,终于到了其原始的解释说明。
现以NM_005715为一个简单的例子(##后面为说明)。
LOCUS      NM_005715              4396 bp    mRNA    linear  PRI 27-JUN-2012    ##说明accession num、序列长度和最后更新日期
DEFINITION  Homo sapiens uronyl-2-sulfotransferase (UST), mRNA.            ##序列所在基因功能描述
ACCESSION  NM_005715                ##accession num
VERSION    NM_005715.2  GI:194578911      ##accession版本号和所在基因GI号
KEYWORDS    .
SOURCE      Homo sapiens (human)        ##序列来源,即序列所在物种
  ORGANISM  Homo sapiens            ##序列物种生物学分类
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE  1  (bases 1 to 4396)          ##参考文献序号
  AUTHORS  Uher,R., Perroud,N., Ng,M.Y., Hauser,J., Henigsberg,N., Maier,W.,            ##参考文献作者
            Mors,O., Placentino,A., Rietschel,M., Souery,D., Zagar,T.,
buchi            Czerski,P.M., Jerman,B., Larsen,E.R., Schulze,T.G., Zobel,A.,
            Cohen-Woods,S., Pirlo,K., Butler,A.W., Muglia,P., Barnes,M.R.,
            Lathrop,M., Farmer,A., Breen,G., Aitchison,K.J., Craig,I.,
            Lewis,C.M. and McGuffin,P.
  TITLE    Genome-wide pharmacogenetics of antidepressant response in the    ##参考文献标题
            GENDEP project
  JOURNAL  Am J Psychiatry 167 (5), 555-564 (2010)                          ##参考文献发表的杂志
  PUBMED  20360315                ##在pubmed数据库中序号
  REMARK    GeneRIF: Clinical trial and genome-wide association study of
            gene-disease association, gene-environment interaction, and
            pharmacogenomic / toxicogenomic. (HuGE Navigator)
REFERENCE  2  (bases 1 to 4396)
  AUTHORS  Trynka,G., Zhernakova,A., Romanos,J., Franke,L., Hunt,K.A.,
            Turner,G., Bruinenberg,M., Heap,G.A., Platteel,M., Ryan,A.W., de
            Kovel,C., Holmes,G.K., Howdle,P.D., Walters,J.R., Sanders,D.S.,
            Mulder,C.J., Mearin,M.L., Verbeek,W.H., Trimble,V., Stevens,F.M.,
            Kelleher,D., Barisani,D., Bardella,M.T., McManus,R., van Heel,D.A.
            and Wijmenga,C.
  TITLE    Coeliac disease-associated risk variants in TNFAIP3 and REL
            implicate altered NF-kappaB signalling
  JOURNAL  Gut 58 (8), 1078-1083 (2009)
  PUBMED  19240061
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE  3  (bases 1 to 4396)
  AUTHORS  Xu,D., Song,D., Pedersen,L.C. and Liu,J.
  TITLE    Mutational study of heparan sulfate 2-O-sulfotransferase and
            chondroitin sulfate 2-O-sulfotransferase
  JOURNAL  J. Biol. Chem. 282 (11), 8356-8367 (2007)
  PUBMED  17227754
  REMARK    GeneRIF: analysis of differences and similarities various residues
            play in the biological roles of the HS-2OST and CS-2OST enzymes
REFERENCE  4  (bases 1 to 4396)
  AUTHORS  Ohtake,S., Kimata,K. and Habuchi,O.
  TITLE    Recognition of sulfation pattern of chondroitin sulfate by uronosyl
            2-O-sulfotransferase
  JOURNAL  J. Biol. Chem. 280 (47), 39115-39123 (2005)
  PUBMED  16192264
  REMARK    GeneRIF: 2OST transfers sulfate preferentially to the GlcA residue
            located in a unique sequence, -GalNAc(4SO(4))-GlcA-GalNAc(6SO(4))-.
REFERENCE  5  (bases 1 to 4396)
  AUTHORS  Mungall,A.J., Palmer,S.A., Sims,S.K., Edwards,C.A., Ashurst,J.L.,
            Wilming,L., Jones,M.C., Horton,R., Hunt,S.E., Scott,C.E.,
…………
  TITLE    The DNA sequence and analysis of human chromosome 6
  JOURNAL  Nature 425 (6960), 805-811 (2003)
  PUBMED  14574404
REFERENCE  6  (bases 1 to 4396)
  AUTHORS  Kobayashi,M., Sugumaran,G., Liu,J., Shworak,N.W., Silbert,J.E. and
            Rosenberg,R.D.
  TITLE    Molecular cloning and characterization of a human uronyl
            2-sulfotransferase that sulfates iduronyl and glucuronyl residues
            in dermatan/chondroitin sulfate
  JOURNAL  J. Biol. Chem. 274 (15), 10474-10480 (1999)
  PUBMED  10187838
COMMENT    VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AI570697.1, DB496757.1, BC093668.1, AB020316.1 and CA842761.1.